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KMID : 1140120050100010054
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Cancer Prevention Research
2005 Volume.10 No. 1 p.54 ~ p.59
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Ethanol Extract of Inonotus Obliquus Shows Antigenotoxic Effect on Hydrogen Peroxide Induced DNA Damage in Human Lymphocytes
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Park Eun-Ju
Jeon Kyung-Im
Byun Boo-Hyeong
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Abstract
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The aim of this study was to evaluate the effect of ethanol extract of Inonotus obliquus (Chaga mushroom) on oxidative DNA damage induced by H2O2 in human lymphocytes. Cells incubated in medium with 1 % DMSO (negative control) or with various concentration of Inonotus obliquus extracts (6.25, 12.5, 25, 50, 100μg/ml) for 30 min at 37oC were further treated with H2O2 (200μM) as an oxidative stimulus for 5 min on ice. Cell viability measured by trypan blue exclusion test was above 95% for all treatments. Oxidative damage was evaluated by Comet assay and quantified as % tail DNA. An increased oxidative DNA damage by H2O2 was significantly inhibited by pre-incubating with 6.25, 12.5, 25, 50, 100μg/ml of Inonotus obliquus extracts by 46, 52, 69, 70 and 80%, respectively. When human lymphocytes were post-incubated with Inonotus obliquus for 30 min after exposure to H2O2 or incubated with Inonotus obliquus and H2O2 simultaneously, the antigenotoxic effect of Inonotus obliquus was not changed. These results indicate that Inonotus obliquus supplementation to human lymphocytes could inhibite or repair H2O2 induced damage to cellular DNA, supporting a protective effect of Inonotus obliquus against oxidative damage. (Cancer Prev Res 10, 54-59, 2005)
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KEYWORD
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Inonotus obliquus extract, Comet assay, Human Lymphocytes, Oxidative DNA damage
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